Part:BBa_K106003:Design

Sir2, Aar1 BD part

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 820
  Illegal BamHI site found at 258
  Illegal BamHI site found at 806
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Since this is a BD part, there is no ATG but it does include a stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.

Source

Yeast genome, with two internal AarI sites mutagenized (conservative).